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Preparation of standard solutions — Preparation of Standard stock Solution
Step-1:
Weighed and transferred (10 mg) of NDBA, (10 mg) of NDPA, (10 mg) of NEIPA and (10 mg) of NDIPA standard into an individual (10 mL) volumetric flask containing (5 mL) of methanol and vortex to dissolve. Diluted up to the mark with methanol and mixed well.
Step-2:
Transferred (0.11 mL) of NDBA, (0.11 mL) of NDPA, (0.11 mL) of NEIPA, (0.11 mL) of NDIPA (Step-1) standard stock solution into a (50 mL) volumetric flask containing (20 mL) of diluent and vortex to dissolve. Diluted up to the mark with diluent and mixed well.
Step-3:
Transferred (0.5 mL) of (Step-2) standard stock solution into a (25 mL) volumetric flask containing (10 mL) of diluent and vortex for (2 minutes). Diluted up to the mark with diluent and mixed well.